Koronavirus novel milik genus B.COVID-19 mangrupikeun panyakit inféksi saluran pernapasan akut.Jalma umumna rentan.Ayeuna, pasien anu katépaan ku novel coronavirus mangrupikeun sumber utama inféksi;jalma anu katépaan asimtomatik ogé tiasa janten sumber inféksi.Dumasar kana panyelidikan epidemiologis ayeuna, jaman inkubasi nyaéta 1 dugi ka 14 dinten, kalolobaanana 3 dugi ka 7 dinten.Manifestasi utama kalebet muriang, kacapean sareng batuk garing.Nasal kamacetan, irung runny, nyeri tikoro, myalgia sarta diare kapanggih dina sababaraha kasus.
Kit ieu dianggo pikeun deteksi kualitatif in vitro novel coronavirus 2019-nCov dina spésimén pernapasan kalebet swab oropharyngeal, sputum, cairan lavage bronchoalveolar sareng swab nasopharyngeal.Set primer sareng usik anu dilabélan FAM dirancang pikeun deteksi khusus gén ORFlab 2019-nCov.Panyilidikan anu dilabélan VIC pikeun gen N 2019-nCov.Gén RNase P Manusa anu diekstrak sakaligus sareng sampel tés nyayogikeun kontrol internal pikeun ngonfirmasi prosedur ékstraksi nukléat sareng integritas réagen.Probe nargétkeun gén RNase P manusa dilabélan ku CY5.
| Komponén | 50 tés / kit |
| RT-PCR Réaksi Campur Réagen | 240μL × 1 tabung |
| Énzim Campur Réagen | 72μL × 1 tabung |
| Panyilidikan primer 2019-nCoV | 48μL × 1 tabung |
| Kontrol Positip | 200 μL × 1 tabung |
| Kontrol négatip | 200 μL × 1 tabung |
Sensitipitas: 200 eksemplar/mL.
Spésifikasi: Henteu aya réaksi silang sareng SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63 sareng HIN1, H3N2, H5N1, H7N9, Influenza B, Parainfluenza Virus (123), Rhinovirus (A). ,B,C), Adenovirus (1,2,3,4,5,7,55), Human interstitial pneumovirus, Human metapneumovirus, EBv, Measles virus, Human cytomegalic virus, Rota virus, Norovirus, Mumps virus, Varicella Zoster Virus , Mycoplasma pneumonia, Chlamydia pneumonia, Legionella, Bordetella pertussis, Haemophilus influenza, Staplhylococcus Aureus, Streptococcus Pneumonia, Streptococcus pyogenes, Klebsiella pneumonia, Tuberculous bacillus, Aspergillus fumigatus, Candida albicans, Cryptococcus glabrataform.
Precision: CV ≤5%.
Sistem PCR Real-Time: Aehealth Diagenex AL, ABI 7500, ViiATM7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0.Bio-Rad CEX96 ToélTMSLAN-96S.SLAN-96P